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axioimager a2 fluorescence microscope  (Carl Zeiss)


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    Structured Review

    Carl Zeiss axioimager a2 fluorescence microscope
    Axioimager A2 Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioimager+a2/axio+microscope+observer+z1+%CE%B2/pmc12149256-112-21-20
    Average 90 stars, based on 1 article reviews
    axioimager a2 fluorescence microscope - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: APJ+ cells in the SHF contribute to the cells of aorta and pulmonary trunk through APJ signaling.
    Article Snippet: Outflow tract (OFT) develops from cardiac progenitor cells in the second heart field (SHF) domain.. APJ, a GProtein Coupled Receptor, is expressed by cardiac progenitors in the SHF.. By lineage tracing APJþSHF cells, we show that these cardiac progenitors contribute to the cells of OFT, which eventually give rise to aorta and pulmonary trunk/artery upon its morphogenesis.

    Article Title: SMC5/6 Promotes Replication Fork Stability via Negative Regulation of the COP9 Signalosome
    Article Snippet: The signal was detected using Clarity Western ECL Substrate (Bio-Rad, 170-5061) and imaged using the Syngene XR5 system and GeneSys V1.5.2 (Syngene, Bengaluru, Karnataka, India). .. Images were captured using a Zeiss Cell Observer Z1 fluorescence microscope linked to an ORCA-Flash 4.0 CMOS camera (Hamamatsu, Shizuoka, Japan), or a Zeiss AxioImager A2 linked to an AxioCam ERc 5s camera (Zeiss, Oberkochen, Germany). .. Images were analyzed and processed using ZEN 2012 blue edition imaging software (V1.1.1.0, Zeiss).

    Fluorescence:

    Article Title: APJ+ cells in the SHF contribute to the cells of aorta and pulmonary trunk through APJ signaling.
    Article Snippet: Outflow tract (OFT) develops from cardiac progenitor cells in the second heart field (SHF) domain.. APJ, a GProtein Coupled Receptor, is expressed by cardiac progenitors in the SHF.. By lineage tracing APJþSHF cells, we show that these cardiac progenitors contribute to the cells of OFT, which eventually give rise to aorta and pulmonary trunk/artery upon its morphogenesis.

    Article Title: SMC5/6 Promotes Replication Fork Stability via Negative Regulation of the COP9 Signalosome
    Article Snippet: The signal was detected using Clarity Western ECL Substrate (Bio-Rad, 170-5061) and imaged using the Syngene XR5 system and GeneSys V1.5.2 (Syngene, Bengaluru, Karnataka, India). .. Images were captured using a Zeiss Cell Observer Z1 fluorescence microscope linked to an ORCA-Flash 4.0 CMOS camera (Hamamatsu, Shizuoka, Japan), or a Zeiss AxioImager A2 linked to an AxioCam ERc 5s camera (Zeiss, Oberkochen, Germany). .. Images were analyzed and processed using ZEN 2012 blue edition imaging software (V1.1.1.0, Zeiss).

    Expressing:

    Article Title: Niche cells regulate primordial germ cell quiescence in response to basement membrane signaling
    Article Snippet: .. GFP::H2B expression from sygl-1p::gfp::H2B was quantified in the PGC nucleus by capturing images of control and SGP-ablated embryos with equal exposure settings using a Zeiss AxioImager A2 descibed above. ..

    Pyrolysis Gas Chromatography:

    Article Title: Niche cells regulate primordial germ cell quiescence in response to basement membrane signaling
    Article Snippet: .. GFP::H2B expression from sygl-1p::gfp::H2B was quantified in the PGC nucleus by capturing images of control and SGP-ablated embryos with equal exposure settings using a Zeiss AxioImager A2 descibed above. ..

    Control:

    Article Title: Niche cells regulate primordial germ cell quiescence in response to basement membrane signaling
    Article Snippet: .. GFP::H2B expression from sygl-1p::gfp::H2B was quantified in the PGC nucleus by capturing images of control and SGP-ablated embryos with equal exposure settings using a Zeiss AxioImager A2 descibed above. ..

    Plasmid Preparation:

    Article Title: HD-ZIP IV genes are essential for embryo initial cell polarization and the radial axis formation in Arabidopsis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Escherichia coli (DH5a) N/A N/A Agrobacterium tumefaciens (GV3101/pMP90) N/A N/A Chemicals, peptides, and recombinant proteins Chloral hydrate Nacalai tesque Cat#07922-62 Glycerol Nacalai tesque Cat#17017-93 Nitsch basal salt mixture Duchefa Cat#N0223 Trehalose dihydrate Wako Pure Chemical Cat#206-18455 MES Dojindo Cat#345-01625 Gamborg’s vitamin solution Sigma-Aldrich Cat#G1019 Experimental models: Organisms/strains Arabidopsis: Col-0 NASC N60000 Arabidopsis: hdg11-1 Nakamura et al.12 SAIL_865 G09 Arabidopsis: hdg12-2 Nakamura et al.12 SALK_127261 Arabidopsis: atml1-3 Ogawa et al.14 SALK_033408 Arabidopsis: pdf2-3 Kamata et al.41 GK-201A01 Arabidopsis: pdf2-4 Kamata et al.41 SAIL_70_G06 Arabidopsis: HDG11p::NLS-Venus Ueda et al.10 N/A Arabidopsis: HDG12p::NLS-Venus Ueda et al.10 N/A Arabidopsis: AtML1p::H2B-Clover This paper N/A Arabidopsis: PDF2p::H2B-Clover This paper N/A Arabidopsis: PDF1p::H2B-Clover This paper N/A Arabidopsis: SHRp::H2B-Clover This paper N/A Arabidopsis: WOX2p::H2B-tdTomato This paper N/A Arabidopsis: WOX8delta-Venus Ueda et al.16 N/A Arabidopsis: DRNp::erGFP Cole et al.19 N/A Arabidopsis: DRNp::erGFP and WOX2p::mTur-LTI This paper N/A Arabidopsis: WOX2p::LTI-tdTomato and WOX2p::H2B-GFP Gooh et al.23 N/A Arabidopsis: HDG11p::HDG11-YFP This paper N/A Arabidopsis: HDG11p::PDF2-YFP This paper N/A Arabidopsis: DD45p::HDG11-YFP This paper N/A Arabidopsis: HTR10p::HDG11-YFP Ueda et al.10 N/A Recombinant DNA Plasmid: HDG11p::NLS-Venus Ueda et al.10 N/A Plasmid: HDG12p::NLS-Venus Ueda et al.10 N/A Plasmid: AtML1p::H2B-Clover This paper MU1861 Plasmid: PDF2p::H2B-Clover This paper MU2303 Plasmid: PDF1p::H2B-Clover This paper MU1850 Plasmid: SHRp::H2B-Clover This paper MU2251 Plasmid: WOX2p::H2B-tdTomato This paper MU1933 Plasmid: WOX8delta-Venus Ueda et al.16 N/A Plasmid: DRNp::erGFP Cole et al.19 N/A Plasmid: WOX2p::mTur-LTI This paper MU2329 Plasmid: WOX2p::LTI-tdTomato Gooh et al.23 N/A (Continued on next page) e1 Current Biology 34, 1–11.e1–e4, October 21, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Plasmid: WOX2p::H2B-GFP Gooh et al.23 N/A Plasmid: HDG11p::HDG11-YFP This paper MU2383 Plasmid: HDG11p::PDF2-YFP This paper MU2388 Plasmid: DD45p::HDG11-YFP This paper MU1051 Plasmid: HTR10p::HDG11-YFP Ueda et al.10 N/A pPZP221 Hajdukiewicz et al.42 N/A pMDC99 Curtis and Grossniklaus43 N/A pMDC100 Curtis and Grossniklaus43 N/A Software and algorithms ImageJ/Fiji National Institute of Health https://imagej.nih.gov/ij/index.html R version 4.2.2 R Core team https://www.r-project.org/ Cellpose version 2.0 Stringer et al.44 https://github.com/MouseLand/cellpose Python version 3.11 Python Software Foundation https://www.python.org Other AxioImager A2 Zeiss N/A CV1000 Yokogawa Electric Cat#CV1000-SP84 CSU-W1 Yokogawa Electric N/A A1R MP Nikon Cat#A1RsiMP(1080) Ti-E-TIRF Mai Tai DeepSee Spectra-Physics N/A 35-mm glass-bottom dish Matsunami Glass Cat#D111300 ..

    Software:

    Article Title: HD-ZIP IV genes are essential for embryo initial cell polarization and the radial axis formation in Arabidopsis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Escherichia coli (DH5a) N/A N/A Agrobacterium tumefaciens (GV3101/pMP90) N/A N/A Chemicals, peptides, and recombinant proteins Chloral hydrate Nacalai tesque Cat#07922-62 Glycerol Nacalai tesque Cat#17017-93 Nitsch basal salt mixture Duchefa Cat#N0223 Trehalose dihydrate Wako Pure Chemical Cat#206-18455 MES Dojindo Cat#345-01625 Gamborg’s vitamin solution Sigma-Aldrich Cat#G1019 Experimental models: Organisms/strains Arabidopsis: Col-0 NASC N60000 Arabidopsis: hdg11-1 Nakamura et al.12 SAIL_865 G09 Arabidopsis: hdg12-2 Nakamura et al.12 SALK_127261 Arabidopsis: atml1-3 Ogawa et al.14 SALK_033408 Arabidopsis: pdf2-3 Kamata et al.41 GK-201A01 Arabidopsis: pdf2-4 Kamata et al.41 SAIL_70_G06 Arabidopsis: HDG11p::NLS-Venus Ueda et al.10 N/A Arabidopsis: HDG12p::NLS-Venus Ueda et al.10 N/A Arabidopsis: AtML1p::H2B-Clover This paper N/A Arabidopsis: PDF2p::H2B-Clover This paper N/A Arabidopsis: PDF1p::H2B-Clover This paper N/A Arabidopsis: SHRp::H2B-Clover This paper N/A Arabidopsis: WOX2p::H2B-tdTomato This paper N/A Arabidopsis: WOX8delta-Venus Ueda et al.16 N/A Arabidopsis: DRNp::erGFP Cole et al.19 N/A Arabidopsis: DRNp::erGFP and WOX2p::mTur-LTI This paper N/A Arabidopsis: WOX2p::LTI-tdTomato and WOX2p::H2B-GFP Gooh et al.23 N/A Arabidopsis: HDG11p::HDG11-YFP This paper N/A Arabidopsis: HDG11p::PDF2-YFP This paper N/A Arabidopsis: DD45p::HDG11-YFP This paper N/A Arabidopsis: HTR10p::HDG11-YFP Ueda et al.10 N/A Recombinant DNA Plasmid: HDG11p::NLS-Venus Ueda et al.10 N/A Plasmid: HDG12p::NLS-Venus Ueda et al.10 N/A Plasmid: AtML1p::H2B-Clover This paper MU1861 Plasmid: PDF2p::H2B-Clover This paper MU2303 Plasmid: PDF1p::H2B-Clover This paper MU1850 Plasmid: SHRp::H2B-Clover This paper MU2251 Plasmid: WOX2p::H2B-tdTomato This paper MU1933 Plasmid: WOX8delta-Venus Ueda et al.16 N/A Plasmid: DRNp::erGFP Cole et al.19 N/A Plasmid: WOX2p::mTur-LTI This paper MU2329 Plasmid: WOX2p::LTI-tdTomato Gooh et al.23 N/A (Continued on next page) e1 Current Biology 34, 1–11.e1–e4, October 21, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Plasmid: WOX2p::H2B-GFP Gooh et al.23 N/A Plasmid: HDG11p::HDG11-YFP This paper MU2383 Plasmid: HDG11p::PDF2-YFP This paper MU2388 Plasmid: DD45p::HDG11-YFP This paper MU1051 Plasmid: HTR10p::HDG11-YFP Ueda et al.10 N/A pPZP221 Hajdukiewicz et al.42 N/A pMDC99 Curtis and Grossniklaus43 N/A pMDC100 Curtis and Grossniklaus43 N/A Software and algorithms ImageJ/Fiji National Institute of Health https://imagej.nih.gov/ij/index.html R version 4.2.2 R Core team https://www.r-project.org/ Cellpose version 2.0 Stringer et al.44 https://github.com/MouseLand/cellpose Python version 3.11 Python Software Foundation https://www.python.org Other AxioImager A2 Zeiss N/A CV1000 Yokogawa Electric Cat#CV1000-SP84 CSU-W1 Yokogawa Electric N/A A1R MP Nikon Cat#A1RsiMP(1080) Ti-E-TIRF Mai Tai DeepSee Spectra-Physics N/A 35-mm glass-bottom dish Matsunami Glass Cat#D111300 ..



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